From next month, those under 16 years old can be fined or even jailed for riding an electric scooter without adult supervision.
Cyclists and users of personal mobility devices will also be banned from holding and using their mobile phones while riding.
In addition, a code of conduct will be introduced to advise pedestrians on the safe practices.
The measures, which were proposed by the Active Mobility Advisory Panel (AMAP) late last year, are part of the Government's changes to the Active Mobility Act passed in February to improve safety on the use of devices such as bicycles and electric scooters.
Other measures that have been implemented include tougher penalties for errant users and requiring retailers to send e-scooters for safety inspections.
The various measures were proposed and introduced in response to a growing number of fires and accidents involving e-scooters last year.
There was also a growing number of active mobility offences, with about 4,900 offences detected last year.